Journal: Molecular cancer research : MCR

Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis

doi: 10.1158/1541-7786.MCR-20-0413

Figure Lengend Snippet: (A) Western Blot analysis of emerin in primary mammary epithelial cells, MCF10A cells, MDA-231 cells and MDA-157 cells. (B) Western Blot quantification of emerin expression for each cell line. Emerin expression was normalized to tubulin and primary mammary epithelial cells. Error bars represent standard error. (n=3) ****p-value < 0.0001, unpaired t-test. (C) Nuclear area for MCF10A (n=116, blue), MDA-231 (n=237, green) and MDA-157 (n=342, red) cells. Error bars represent standard error. ****p-value < 0.0001, unpaired t-test (D) Representative DAPI (blue) images of MCF10A, MDA-231, and MDA-157 cells. Scale bars: 100 μm. (E) Nuclear circularity for MCF10A (n=75, blue), MDA-231 (n=301, green), and MDA-157 (n=237, red) cells. Error bars represent standard error. ****p-value < 0.0001, unpaired t-test

Article Snippet: Primary mammary epithelial cells were grown in mammary epithelial cell basal medium (ATCC, cat#: PCS-600–030) with all the components from the mammary epithelial cell growth kit (ATCC, cat#: PCS-600–040).

Techniques: Western Blot, Expressing

Journal: Life (Basel, Switzerland)

Article Title: Eugenol Induces Apoptosis in Tongue Squamous Carcinoma Cells by Mediating the Expression of Bcl-2 Family.

doi: 10.3390/life13010022

Figure Lengend Snippet: Figure 5. Relative fold change expression of mRNA of proapoptotic (Bax and Bad) and anti-apoptotic (Bcl-2) markers in human gingival fibroblast (HGF) (a) and in tongue squamous carcinoma cells (SCC-4) (b) 72 h after exposure to Eug 0.5 mM. mRNA expression levels normalized to 18 S expression. Mean values ± SD of three independent experiments are presented. One-way ANOVA with Dunnett’s multiple comparisons post hoc test was used to identify the statistical differences (* p < 0.05 and ** p < 0.01).

Article Snippet: Fibroblast Basal Medium (ATCC® PCS-201-030TM), Fibroblast Growth Kit-Low Serum (ATCC® PCS-201-041TM), and DMEM: F-12 Medium (ATCC® 30-2006TM) were purchased from ATCC (American Type Culture Collection, Lomianki, Poland).

Techniques: Expressing

Journal: Nature communications

Article Title: LEADeR role of miR-205 host gene as long noncoding RNA in prostate basal cell differentiation.

doi: 10.1038/s41467-018-08153-2

Figure Lengend Snippet: Fig. 5 LEADR regulates basal–luminal differentiation. a Bright-field images showing morphological changes occurring in RWPE-1 (left) and PrEC (right) cells (day 3) upon LEADR silencing by siLEADR (top) or gapLEADR (bottom). Scale bar, 50 µm. Full-size images reported in Supplementary Fig. 4c. b qRT-PCR showing changes in basal and luminal cytokeratins in RWPE-1 or PrEC cells upon LEADR silencing by siLEADR (top) or gapLEADR (bottom). Mean + s.d. plotted. c Western blots showing changes in basal/luminal cytokeratins in RWPE-1 (left) or PrEC (right) cells upon LEADR silencing by siLEADR (top) or gapLEADR (bottom). Vinculin used as loading control. d Immunofluorescence showing cytoplasmic re-localization of p63 (green) upon LEADR silencing in RWPE-1 cells by siLEADR (top) or gapLEADR (bottom). Nuclei counterstained with DAPI (blue). Scale bar, 50 µm. Full-size images are reported in Supplementary Fig. 4d. e Western blots showing changes in Androgen Receptor (AR) expression in PrEC cells upon LEADR silencing by siLEADR (left) or gapLEADR (right). GAPDH used as loading control. f Immunofluorescence showing AR (red) expression in PrEC cells upon LEADR silencing by siLEADR, in the presence or absence of simultaneous DHT stimulation. Nuclei counterstained with DAPI (blue). Scale bar, 50 µm. Full-size images reported in Supplementary Fig. 4g. g ELISA-based quantification of PSA in the conditioned media of PrEC cells silenced for LEADR expression by siLEADR (±DHT). Mean + s.d. (n = 2) plotted. h Heatmap (bottom) of the normalized enrichment score (NES) for luminal and basal custom gene sets (obtained from gene- expression data of frankly basal and luminal prostate cells from the indicated datasets) in RWPE-1 cells upon knockdown of LEADR and/or miR-205 (the latter abrogated using an antisense LNA-modified oligomer, LNA205). Bar plot of differential luminal/basal NES is reported (top). Mean + s.d. (n = 4) plotted. i Plot showing propensity of wild type (wt), LEADR-overexpressing and CRISPRed RWPE-1 cells to differentiate toward luminal phenotype (measured by qRT-PCR and expressed as KRT18/KRT5 ratio) upon culturing in media with increasing differentiative potential (n = 3). ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001 (Student’s t test). Source data are provided as a Source Data file, together with n of all experiments

Article Snippet: The normal primary prostate epithelial cells (PrEC) were grown in PrEC basal medium (ATCC; PCS-440-030) supplemented with PrEC growth kit (ATCC; PCS-440-040).

Techniques: Quantitative RT-PCR, Western Blot, Control, Expressing, Enzyme-linked Immunosorbent Assay, Gene Expression, Knockdown